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. 2014 Jul 3;2014:787023. doi: 10.1155/2014/787023

Figure 7.

Figure 7

Knockdown of RIG-I and TLR3 reduces the nuclear translocation of AP-1 and NF-κB. (a) BV-2 cells were transfected with RIG-I shRNA (RIG-I KD) or negative control shRNA (CTR) and then infected with JEV at an MOI of 1. AP-1 and NF-κB in the nucleus were detected with western blotting at 5 hpi. (d) BV-2 cells were transfected with TLR3 shRNA (TLR3 KD) or negative control shRNA (CTR) and then infected with JEV at an MOI of 1. AP-1 and NF-κB in the nucleus were detected with western blotting at 5 hpi. (b, c, e, f) The protein levels were quantified with immunoblot scanning and normalized to the amount of lamin A or β-tubulin. Error bars represent the standard deviation of results from three independent assays (**P < 0.01; ***P < 0.001).