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. 2013 Jun 17;123(7):3084–3098. doi: 10.1172/JCI64401

Figure 10. SIRT1 interacts and deacetylates p53 in renal epithelial cells.

Figure 10

(A) The interaction between SIRT1 and p53 in WT and Pkd1-null MEK cells was examined by co-IP using either anti-SIRT1 or anti-p53 antibody. IgG was used as a negative control. (B) Western blot analysis of p53 and acetyl-p53 expression from whole cell lysates of WT MEK, Pkd1-null MEK, PH2, and PN24 cells. Acetyl-p53 expression was decreased in Pkd1-null MEK versus WT MEK cells, whereas p53 expression was almost at same levels in these cells. (C and D) Knockdown of SIRT1 with siRNA or inhibition of SIRT1 with nicotinamide increased p53 acetylation, but did not affect p53 expression, in Pkd1-null MEK cells and PN24 cells that were (C) transfected with SIRT1 siRNA for 48 hours or (D) treated with 10 mM nicotinamide for 24 hours. *P 0.05, **P 0.01.