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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Jan 5;21(2):143–151. doi: 10.1038/nsmb.2740

Figure 5.

Figure 5

WT-EttA inhibits synthesis of the first peptide bond at low ATP/ADP ratio. (a) Room temperature in vitro translations with or without 0.6 mM ADP and 1.2 mM ATP were analyzed by eTLC. Reactions, conducted as in Fig. 4a but with the 70S IC desalted in Polymix Buffer, contained 0.3 mM GTP, 0.6 µM 70S ribosomes, and when indicated 3.5 µM of an EttA variant added in parallel with the elongation factors, Phe-tRNAPhe, and Lys-tRNALys. (b) Quantification of products in the autoradiograms in panel a using ImageQuant software, with error bars representing standard error of the mean.