Figure 1. The enzymatic reaction catalyzed by Sirt1.
Sirt1 catalyzes the deacetylation of several proteins by consuming nicotinamide adenine dinucleotide (NAD+), generating nicotinamide (NAM) and 2′-O-Acetyl-ADP-Ribose. NAM is recycled back into NAD+ by the enzymes nicotinamide phosphorybosyltransferase (NAMPT), nicotinamide mononucleotide adenylyltransferase (NMNAT), and the nicotinamide mononucleotide (NMN) intermediate.