Table 3.

FTIR spectroscopy and ligand binding kinetics parameters measured for MbCNR complexes with L29 mutations A, and F.^a

	FTIR spectra data			CNR binding data
MbCNR complex	in νCN cm−1	out νCN cm−1	Fin	Fgem	k′ μM−1s−1	k s−1	Ka μM−1
wt
CNC1	2083	2139	0.60	0.80	0.12b	4.3b	0.028b
CNC2	2065	2106	0.82	0.95	0.074b	0.27b	0.27b
CNC3	2077	2112	0.69	0.79	0.043b	0.33b	0.13b
CNC4	2083	2113	0.47	0.63	0.029b	0.60b	0.048b
CNC5	2081	2131	0.68	0.89	0.030c	0.44c	0.069c
L29A
CNC1	2098	2154	0.53	0.62	0.58d	7.7d	0.075d
CNC2	2075	2119	0.62	0.97	0.70d	0.63d	1.1d
CNC3	2093	2123	0.63	0.97	0.45	0.023	20
CNC4	2089	2126	0.66	0.98	2.0	0.020	100
CNC5	2098	2134	0.68
L29F
CNC1	2084	2133	0.38	0.29	0.19d	57d	0.0033d
CNC2	2079	2108	0.71	0.44	0.090d	4.2d	0.021d
CNC3	2093	2127	0.76	0.42
CNC4	2099	2123	0.69	0.28
CNC5	2098	2124	0.71

^aDetails regarding the parameters are presented in Table 1. Blank cells indicate parameters that have not been measured.

^bFrom (11).

^cValues are for native sperm whale Mb and averages from (10, 12).

^dFrom (7).