Table 4.

FTIR spectroscopy and ligand binding kinetics parameters measured for MbCNR complexes with I107 mutations A, and W.^a

	FTIR spectra data			CNR binding data
MbCNR complex	in νCN cm−1	out νCN cm−1	Fin	Fgem	k′ μM−1s−1	k s−1	Ka μM−1
wt
CNC1	2083	2139	0.60	0.80	0.12b	4.3b	0.028b
CNC2	2065	2106	0.82	0.95	0.074b	0.27b	0.27b
CNC3	2077	2112	0.69	0.79	0.043b	0.33b	0.13b
CNC4	2083	2113	0.47	0.63	0.029b	0.60b	0.048b
CNC5	2081	2131	0.68	0.89	0.030c	0.44c	0.069c
CNC6	2078	2137	0.90	0.97	0.037c	0.15c	0.25c
I107A
CNC1	2089	2141	0.59	0.73	0.091	2.3	0.040
CNC2	2067	2147	0.91	0.92	0.12	0.15	0.80
CNC3	2083	2141	0.87	0.99	0.13	0.025	5.2
CNC4	2081	2143	0.80	0.99	0.19	0.0088	22
CNC5	2079	2144	0.76	0.99
I107W
CNC1	2096	2143	0.49	0.87	0.18	0.21	0.86
CNC2	2066	2147	0.85	0.94	0.16	0.025	6.4
CNC3	2077	2139	0.82	0.96	0.017	0.029	0.59
CNC4	2077	2141	0.78	0.87	0.062	0.044	1.4
CNC5	2073	2137	0.57	0.91
CNC6	2074	2136	0.68	0.97

^aDetails regarding the parameters are presented in Table 1. NA = Not Applicable. Blank cells indicate parameters that have not been measured.

^bFrom (11).

^cValues are for native sperm whale Mb and averages from (10, 12).