Figure 6.

LmES induce GrB production by CD4⁺CD25⁺CD127^dim/− regulatory T in HZCL. CD4⁺CD25⁺CD127^dim/− regulatory T cells were purified from peripheral blood of 4 HZCL and 3 HC and stimulated with LmES (10 μg/mL) in vitro in the presence of mitomycin C-treated PBMCs (APCs) at different Effector/APC ratios for 5 days. Culture supernatants were used to determine GrB concentration. Results are expressed as the mean ± standard error of the mean of levels. Statistical analysis: *P < 0.05 when comparing HZCL to HC.