Figure 7.

Electrophoresis mobile shift assay in porcine OPN promoter CpG sites. (a) Using CpG1 contained DNA element binding with SH-SY5Y nuclear extract. Methylated and unmethylated competitors were used as 2X, 5X, and 10X concentration than isotope labeled probe. (b) CpG 13th probe of OPN promoter binding with HEK-293 nuclear extract. (c) CpG 20th of OPN promoter element binding with HEK-293 nuclear extract. There was no difference between methylated or unmethylated competitors in competition experiment.