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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Struct Biol. 2014 Apr 12;186(2):265–272. doi: 10.1016/j.jsb.2014.04.003

Figure 2.

Figure 2

The single filament IHRSR 3D reconstruction method (SF-IHRSR). This figure, based on the IHRSR method (Egelman, 2000), highlights the three modifications we have implemented: (1) generating out-of-plane angle and Y shift projections (Alamo et al., 2008) instead of only azimuthally rotation projections (i.e. 4095 instead of 90 reference projections); (2) using a subset of the tilt series images from an electron tomogram of a single filament instead of using thousands of segments from many thick filaments; and (3) applying a Fermi band pass filter to each image before the back projection (Pinto et al., 2012). The Egelman programs and Spider operators used in each case are enclosed between parenthesis and square brackets respectively.