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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Cancer Genet. 2014 Mar 18;207(4):133–140. doi: 10.1016/j.cancergen.2014.03.004

Figure 1. Determination of the breakpoints of the t(3;8).

Figure 1

(A) t(3;8) breakpoint mapping on chromosome 8 with DNA from der(3)t(3;8) somatic cell hybrid. On the left is the ideogram of chromosome 8 with a screenshot from the UCSC genome browser below in the region containing the PATRR in the RNF 139 gene. On the right are the relevant PCRs. (B) t(3;8) breakpoint mapping on chromosome 3 with DNA from der(3)t(3;8) somatic cell hybrid. On the left is the ideogram of chromosome 3 with a screenshot from the UCSC genome browser below in the region containing PATRR3 in the FHIT gene. On the right are the relevant PCRs. Lane +, Healthy control; Lane *, der(3)t(3;8) hybrid cell; Lane -, Negative control; Lane M, 1kb+ladder. Sequences surrounding the breakpoints are visualized in the UCSC genome browser. (C) PCR for detection of the breakpoint region of the t(3;8) translocation in a balanced carrier. Primer locations are shown as arrows above the chromosomes. The black bar indicates chromosome 8q, the red bar the chromosome 8 PATRR, the white bar indicates the chromosome 3 PATRR and the grey bar chromosome 3p.. Lane +, Healthy control; Lane *, t(3;8) carrier; Lane −, Negative control; Lane M, 1kb+ladder.