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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Cell Stem Cell. 2014 Jun 5;14(6):810–823. doi: 10.1016/j.stem.2014.04.014

Figure 4. Prolonged fasting promotes IGF-1/PKA dependent hematopoietic regeneration.

Figure 4

(A) PKA-dependent phosphorylation of CREB visualized by ICC in mouse embryonic fibroblast (MEFs) devoid of endogenous IGF-1R (R- cells) or overexpressing human IGF1R (R+ cells). R+ cells were treated with IGF-1 and compared to cells transfected with PKACα siRNA.

(B) Prolonged fasting (PF) reduces both circulating IGF-1 levels and PKA activity in BM cells in mice.

IGF-1 injection blunted the PF-induced (C) reduction of PKA/pCREB,(D) increase in hematopoietic stem cells, (E-H) The chimerism of donor-derived cells in PB and that in the BM was determined 16 weeks after primary and secondary BM transplantation.. n=4 to 8 female mice per group, * p<0.05, **p<0.01 and *** p<0.005, one-way ANOVA.