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. 2014 Jul 17;10(7):e1004490. doi: 10.1371/journal.pgen.1004490

Figure 4. SIRT1 overexpression in skeletal muscle does not affect the fasting response.

Figure 4

(A) Blood glucose and plasma insulin levels in fed or after overnight fasting of WT and Tg-4140 mice (8–10 weeks old, n = 6–10). (B–G) Relative mRNA levels of GLUT4, PDK4, mitofusin, mCPT1, ERRα, and MCAD genes in gastrocnemius muscle of fed and fasted WT and Tg-4140 (10–12 weeks old, n = 4). (H) Western blot analyses in gastrocnemius muscle protein extracts prepared from gastrocnemius muscle of WT and Tg-4140 (10–12 weeks old). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.