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. 2014 Jul 17;9(7):e101338. doi: 10.1371/journal.pone.0101338

Figure 3. mRNA-Seq expression estimates validated with quantitative real time PCR (qRT-PCR) for anthocyanin biosynthetic pathway associated genes.

Figure 3

mRNA-Seq expression estimates are strongly correlated with qRT-PCR for nine genes associated with the anthocyanin biosynthetic pathway for purple- and white-flowered samples from two populations – Savage River (filled triangles = purple petals; open triangles = white petals) and 12 Mile Summit (filled circles = purple petals; open circles = white petals). mRNA-Seq expression estimates are measured in reads per kilobase exon per million uniquely mapped reads (RPKM) and log transformed to fit assumptions of normality. The qRT-PCR data, measured using the ΔCT method, have been linearized and log transformed for comparison to the Illumina expression estimates.