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. 2014 Jul 17;9(7):e101338. doi: 10.1371/journal.pone.0101338

Figure 7. Identification of consistently differentially expressed genes.

Figure 7

Consistently differentially expressed genes were identified by comparing the mean relative difference (RD) between purple- and white-flowered samples from Savage River and 12 Mile Summit to the consistency of the RD estimates between those two populations. RD was calculated for each purple-white pair of samples at a population [RD = (purple RPKM – white RPKM)/max(purple RPKM, white RPKM)]. Consistency is the difference between RD Savage River and RD 12 Mile Summit. We focused on points with RD>0.5 or RD<–0.5 and with consistency less than 0.25 and greater than –0.25 (larger red filled circles). Chalcone synthase is indicated with a red triangle as the most consistently differentially expressed gene with greater expression in purple than white. There are approximately three times more consistently differentially expressed genes with white expression greater than purple (25 genes) compared to the number of consistently differentially expressed genes with purple greater than white (8 genes).