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. 2014 Jul 17;9(7):e101984. doi: 10.1371/journal.pone.0101984

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© 2014 Umeki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Chemical wounds created in rabbit gingiva. (a) Filter paper (5 mm in diameter) soaked with 50% acetic acid attached to rabbit mandibular gingival for 2 minutes. (b) Gingival ulcer formed on the day following wound creation. (B) Histological findings during wound repair at day 6 after wound creation. Spaces between the two arrowheads show ulcerated area without epithelial lining. H-E staining. Scale bars = 500 µm. (C) Sequential changes in ulcerated area. (D) Total duration for complete healing of rabbit gingival ulcer. (E) Number of blood vessels in the subepithelial connective tissue beneath the ulcerated area. Values are mean ± SE from 12 animals per group. *P<0.05, **P<0.01.