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. 2014 Jul 17;9(7):e101984. doi: 10.1371/journal.pone.0101984

Figure 4. Effect of leptin on a human oral mucosal cell line (RT7 cells).

Figure 4

(A) Ob-R mRNA expression in RT7 cells. RNase (+): Total RNA treated with RNase. DNase (+): cDNA treated with DNase. (B) Effect of leptin on the proliferation of RT7 cells. (C) Semi-quantitative RT-PCR analysis. Effect of leptin on the expression of mRNA encoding oral mucosal epithelial cell-related genes (Keratin 4, Keratin 10 and Transglutaminase I) in RT7 cells. RNase (+): Total RNA treated with RNase. DNase (+): cDNA treated with DNase. (D) Real-time quantitative RT-PCR analysis of Keratin 4. Values are mean ± SE (n = 8). (E) Real-time quantitative RT-PCR analysis of Keratin 10. Values are mean ± SE (n = 8). *P<0.05 (F) Real-time quantitative RT-PCR analysis of Transglutaminase I. Values are mean ± SE (n = 8). *P<0.05 (G) Effect of leptin on the migration of RT7 cells. Values are mean ± SE from two independent experiments performed in triplicate. *P<0.05, **P<0.01.