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. 2014 Jul 17;9(7):e102496. doi: 10.1371/journal.pone.0102496

Figure 4. CD147 interaction with vinculin in HCC cells.

Figure 4

(A) CD147 immunoprecipitated with vinculin. Lysates of SMMC-7721 cells were subjected to immunoprecipitation with an anti-CD147 or anti-vinculin antibody pre-bound coupling gel. Vinculin and CD147 were detected in immune complexes by immunoblotting (IB) analysis. Mouse IgG was used as a negative control. (B) The interaction between vinculin and CD147 was analyzed with FRET imaging. Two individual dishes of SMMC-7721 cells were transfected with DsRed1-N1-vinculin or pEGFP-CD147 as the acceptor and donor samples, respectively. Both DsRed1-N1-vinculin and pEGFP-CD147 were transfected into SMMC-7721 cells together as the FRET sample. Using the donor and acceptor samples, CoA and CoB were automatically calculated with FRET software. The correct FRET is representative of the interaction affinity. The FRET ratio is shown on the right.