Figure 2. The effect of endothelium-specific Ass deletion on hemodynamics of 34-week-old conscious male mice.

Black bar: control mice; white bar: Ass-KO^Tie2 mice. Blood pressure was measured in the same mice 2 (panel A) and 3 days (panel B) after catheterization via a femoral artery catheter connected to a pressure transducer. Panel A: mean arterial pressure (MAP) in the basal condition (left) and after a bolus infusion of 200 U bovine arginase 1 via a jugular vein catheter (right). Panel B: mean arterial pressure in the basal condition (left) and after intravenous L-NAME (10 mg/kg) infusion (right). Values are means ± SEM (control animals: arginase 1: n = 7, L-NAME: n = 5; Ass-KO^Tie2 mice: arginase 1: n = 5, L-NAME: n = 4; due to loss of catheter patency, numbers were lower on the 3^rd day). Note that the Y-axis starts at 90 mm Hg.