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. 2014 Jul 17;9(7):e102264. doi: 10.1371/journal.pone.0102264

Figure 3. The effect of endothelium-specific Ass deletion on relaxation responses of saphenous arteries of healthy and diabetic male mice.

Figure 3

Relaxation of PHE (10 µM)-pre-contracted saphenous arteries of 12- (panels A–C) and 34-week-old (panels D–F) healthy and 22-week-old diabetic (panels G–I) male mice to ACh (0.01–10 µM) was determined by wire myography. Black squares: control mice; white circles: Ass-KOTie2 mice. Panels (A, D, G): relaxation responses in the absence of pharmacological inhibitors. Panels (B, E, H): relaxation responses in the presence of INDO (10 µM). Panels (C, F, I): relaxation responses in the presence of both INDO (10 µM) and L-NAME (100 µM). Values are shown as means ± SEM (n = 5–7; for the number of animals per individual experiment see Table 1). *P<0.05 vs. the control, **P<0.01 vs. the control (unpaired t-test).