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. 2014 Jul 17;9(7):e102360. doi: 10.1371/journal.pone.0102360

Figure 6. Nematode resistance test for WRKY33 overexpression lines.

Figure 6

The resistance of overexpression lines of WRKY33 with constitutive 35S promoter (A&D) and syncytia specific promoters Pdf2.1 (B&E) and Miox5 (C&F) was compared to wild type plants after infection with H. schachtii. A–C: Number of male and female nemaodes per cm of root length calculated at 15 dpi setting the wild type as 100%. Asterisks indicate significant differences (*, P<0.05 and **, P<0.01; ANOVA and LSD). The statistical significance was determined by three independent replicates. Values are means ± SE, n = 15. The bar shows standard error for the mean. D–F: Size of female syncytia and female nematodes at 14 dpi. Ten syncytia were selected randomly from three independent replicates (total = 30) and the size of syncytia and associated female nematodes was determined. Data were analysed for significance difference using ANOVA (P<0.05) and LSD. Values are means ± SE.