Figure 3. Cd and Zn tolerance of yeast cells expressing SaMT2.

The Saccharomyces cerevisiae BY4741, Δycf1 and Δzrc1 yeast cells harboring pDR195 (vector control) or pDR195-SaMT2 were grown in liquid SD selective medium. Cultures were adjusted to OD_600nm of 0.1 and serially 10-fold diluted in water. 10 µl aliquots of each dilution were spotted either on SD selective plates or on plates with 30 µM CdCl₂ or 5 mM ZnSO₄. After 3 days of incubation at 30°C, plates were photographed. CK represents the control group.