Figure 1. Leptospiral-induced nephritis in C57BL/6J wild-type and Daf1^−/− mice at 14 and 90 dpi.

A) Survival percentage of WT+PBS (dark rhombus), WT+LIC (white square), DAFKO+PBS (white triangle) and DAFKO+LIC (dark circle) mice. Mice were monitored daily and euthanized by CO₂ overdose at 90 dpi (5–7 mice were used). Mortality of C57BL/6J Daf1^−/− LIC-infected mice at 3 or 4 dpi was unexpected and presented no previous signs. B–C) Histopathologic sections of uninfected (WT+PBS and DAFKO+PBS) and infected C57BL/6J wild-type (WT+LIC) and Daf1^−/− (DAFKO+LIC) kidneys at 14 and 90 dpi assessed by hematoxylin and eosin staining (x200). Lymphomonocytic-rich infiltrates are indicated by arrows. D–E) Inflammation score of uninfected and LIC-infected C57BL/6J wild-type and Daf1^−/− animals. Mean inflammation score is represented by a straight line; *p<0.05, **p<0.01, ***p<0.001, ^ns p>0.05.