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. 2014 Jul 17;9(7):e102831. doi: 10.1371/journal.pone.0102831

Figure 3. Induction of apoptotic cell death by tenovin-6 in gastric cancer cells.

Figure 3

A: Time-course analysis of the expression of p53, its downstream molecule p21Waf/Cip1, and apoptosis-related molecules in gastric cancer cells treated with tenovin-6 (10 µM). Relative intensity of the proteins' expression is shown in Figure S1. Tenovin-6 induced the expression of Ac-p53 and p21Waf/Cip1, but not Bcl-2. DR5 expression was strongly induced by tenovin-6. TRAIL, and cleaved PARP were slightly increased, but FADD expression was not affected. A doublet of DR5 shows its precursor (upper band) and mature isoforms (lower band). B: Four cell lines were treated with tenovin-6 (10 µM) or a vehicle control for 72 h, double-stained with FITC-annexin V and PI, and analyzed by flow cytometry. The statistical significance of differences between groups was evaluated using Student's t-test. * p<0.01; ** p<0.05.