Table 1. Mutations in the Prx2/S8-binding site and their effects on EGFP expression in the ventricle.
Construct | EGFP Expression | |
---|---|---|
P234: | 5′-CTAAATTAGCCCTCCGCTATCAGAGAA | 12% (n = 140) |
P234M1: | 5′-CTAAATTCCCCCTCCGCTATCAGAGAA | 0% (n = 126) |
P234M2: | 5′-CTAAACCAGCCCTCCGCTATCAGAGAA | 0% (n = 130) |
P234M3: | 5′-CTAAATTAGGGCTCCGCTATCAGAGAA | 2% (n = 118) |
P234M4: | 5′-CCCAATTAGCCCTCCGCTATCAGAGAA | 2% (n = 109) |
P234M5: | 5′-CTAAATTAGCCCTCCGCCCTCAGAGAA | 10% (n = 105) |
Underlined: Prx2/S8 consensus binding site. n: total number of microinjections for each construct.