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. 2014 Jul 17;9(7):e102826. doi: 10.1371/journal.pone.0102826

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Rarefaction analysis was performed using the de-noised 454 pyrotag sequencing data on de novo picked OTUs for (A) A-horizon and (B) clay samples. Each curve is the average from experimental replicates (N = 2 libraries for the 2001 method and ORNL 2012 method A-horizon samples; N = 3 libraries for the remainder of the sample/method combinations) from 100 jackknife iterations over each sampling depth. The key for the A-horizon comparison of accessed diversity uses the same color code as for the clay sample comparison.