Table 1. Comparison of Methods by OTU Countsa percentage of OTUs from Dominantly Gram Positive Phyla.
Method | Clay | A-horizon | ||||
OTUCountb | %Firmicutes±sd | %Actinobacteria±sd | OTUCountb | %Firmicutes±sd | %Actinobacteria±sd | |
ORNL 2001 | 233±48 | 0.07±0.06 | 11.47±2.2 | 521±16 | 0.05±0.07 | 15.65±0.07 |
ORNL 2012 | 495±6 | 1.73±1.10 | 7.90±1.31 | 568±60 | 1.00±0.28 | 11.45±3.32 |
PowerLyzer™ | 252±29 | 0.13±0.15 | 6.30±1.76 | 535±23 | 0.07±0.06 | 10.77±2.15 |
Data is from closed-reference picked OTUs, rarefied at 1404 sequences per sample.
Values are averages among replicate extractions.