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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Invest Dermatol. 2014 Mar 4;134(8):2154–2162. doi: 10.1038/jid.2014.124

Figure 4. Treatment of epidermal models with the HDAC inhibitor TSA increases differentiation marker expression after UVB exposure.

Figure 4

a) Organotypic models were unexposed or exposed to 1500 J/m2 UVB prior to lifting to the air-liquid interface, left untreated or treated with DMSO or TSA for 4 hours daily starting 24 hours later, then harvested 4 days after lifting. Numbers represent band intensity fold change comparing unexposed treated and untreated cultures and comparing UVB-exposed treated and untreated cultures after normalization to GAPDH. Immunoblots revealed that TSA treatment helped restore Dsg1, Dsc1, and K10 expression after UVB exposure. b) TSA treatment reduces phosphorylated Erk in UVB- exposed organotypic cultures compared to DMSO treated controls.