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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Cancer Res. 2014 May 15;74(14):3959–3970. doi: 10.1158/0008-5472.CAN-13-3137

Figure 4. Cyclin D1 is required for extranuclear E2-mediated expression and recruitment of Rad51 to nuclear foci in response to DNA damage.

Figure 4

(A) Schematic depicting experimental procedure. Immunofluorescence staining for cyclin D1 and Rad51 with the nucleus identified by DAPI staining. (B) Western blot of MCF-7 cells transduced with three distinct cyclin D1 siRNA and subjected to treatment with E2 (10−8M). (C) Quantitation of Rad51 mRNA abundance determined by QT-PCR. Cells were treated with 10−8M E2 for 24 hours.