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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Cancer Res. 2014 May 15;74(14):3959–3970. doi: 10.1158/0008-5472.CAN-13-3137

Figure 6. Estradiol enhances the DNA damage response of repair factor recruitment to DNA.

Figure 6

(A) Schematic representation of the chimeric fusion proteins in which the DNA damage signaling repair factors, or cyclin D1, were linked to the cherry-lacR-NLS (red). (B)Western blot NIH 2/4 cells and NIH 2/4 cells transduced with an ERα expression plasmid. Immuno-blotting was conducted with antibodies as indicated. (C) Schematic representation of the cell treatment protocol. (D) Confocal immunofluorescence microscopy of NIH 2/4 cells transiently transfected with the DNA damage repair factor or cyclin D1 fused to cherry-lacR-NLS (red). Phosphorylation of γH2AX indicates activation of the DDR. (E) The percentage of cells with γH2AX signal was determined at 24 hours after treatment (E2 10−8M). The data are mean ±SEM for N>50 cells and N>3 separate experiments. (F) Models of E2 on cyclin D1, ATM and NBS1 chromatin recruitment-mediated γH2AX foci.