Table 1.

VWF fragment/FVIII binding constants

VWF fragment	kon (min−1nM−1)	koff (min−1)	KD (nM)
D1-D3	1.7 × 10−2 ± 1.3 × 10−3	7.8 × 10−3 ± 7.7 × 10−4	0.48 ± 0.066
D′D3	1.9 × 10−2 ± 1.3 × 10−3	1.8 × 10−2 ± 8.9 × 10−4*	0.92 ± 0.049*
D′D3-Fc	1.3 × 10−2 ± 1.2 × 10−3	1.9 × 10−2 ± 1.8 × 10−3*	1.5 ± 0.17*
truncD′D3	1.6 × 10−2 ± 1.3 × 10−3	5.5 × 10−2 ± 4.5 × 10−3*	3.8 ± 0.51*

Kinetic constants were determined from 3 to 4 independent binding experiments at 4 FVIII concentrations. Reported values (mean ± standard error of the mean) were averaged from regressions with r² > 0.95.

K_D, dissociation constant = k_off/k_on; k_off, dissociation rate; k_on, association rate.

^*P < .0005 vs D1-D3.