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. 2014 Apr 2;5(10):2918–2933. doi: 10.18632/oncotarget.1577

Figure 3. Influence of miR-424 expression and miR-424 and miR-503 depletion on the effect of T3 on target proteins.

Figure 3

(A) miR-424 levels in untreated and T3-treated cells transfected 36 h before with an expression vector for miR-424 (pSGU-miR424) or the corresponding empty vector. (B) The levels of CHK1, WEE1, CDC25, E2F3, c-MYB, CDK6 and CCND2 were measured by western blot. ERK2 levels were used as a loading control. (C) SK-TRb cells were transfected with anti-miRNAs for miR-424 or miR-503, or with a negative control, and the levels of the corresponding miRNAs were determined in untreated and T3-treated cells 36 h after transfection. (D) Levels of the indicated proteins were analyzed by western blot under the same conditions.