Table 2. Combined results from the qRT-PCR, MSP, and IHC analyses for CADM1.

CADM1
Pat. No.	Sample	qPCR	methylation	IHC-result
BrM-9	BCBM	INT	MET	NEG
BrM-10	BCBM	LOW	MET	NEG
BrM-8	BCBM	INT	HET	NEG
BrM-7	BCBM	LOW	HET	NEG
BrM-5	BCBM	LOW	WT	NEG
BrM-25	BCBM	LOW	WT	NEG
BrM-19	BCBM	LOW	WT	NEG
BrM-1	BCBM	INT	WT	NEG
BrM-16	BCBM	INT	WT	NEG
BrM-18	BCBM	INT	WT	NEG
BrM-22	BCBM	INT	WT	NEG
BrM-2	BCBM	INT	WT	NEG
PT-95	PT	INT	WT	NEG
PT-103	PT	INT	WT	NEG
BrM-13	BCBM	n.d.	WT	NEG
BrM-21	BCBM	INT	HET	WEAK
PT-88	PT	INT	HET	WEAK
BrM-15	BCBM	INT	n.d.	WEAK
BrM-14	BCBM	HIGH	n.d.	WEAK
BrM-24	BCBM	LOW	WT	WEAK
PT-101	PT	INT	WT	STRONG
BrM-11	BCBM	HIGH	WT	STRONG

BCBM: breast cancer brain metastase; PT: primary tumor;

HIGH: CT expression value in upper quartile in all patients analyzed;

LOW: CT expression value in bottom quartile in all patients analyzed n.d.: not defined; WT: wild type; HET: heterozygous methylation;

MET: homozygous methylation; NEG: negative protein staining