Figure 9. MFP treatment increased tumor remodeling and the amount of functional vessels in mammary carcinomas showing higher levels of PRA than PRB.

A. 59-2-HI tumors growing in BALB/c-GFP mice were treated or not for 5 days with MFP (10 mg/kg/day). An increase in stromal cells (green cells; GFP) was observed in MFP-treated tumors. Nuclei were counterstained with PI (red); bar = 300 µm. B. Similar experiments with tumors growing in BALB/c mice. An increase in CD31 positive cells (green staining; FITC) was observed in MFP-treated tumors. Nuclei were counterstained with PI; bar = 300 µm. C. TL were iv injected in mice bearing tumors treated as described in B. Mice were euthanized and tumors processed for IF studies. PI was used for nuclear staining. Increased total lectin fluorescence (green) was observed in MFP-treated tumors; bar = 300 µm. D. Co-localization between TL (green) and CD31 immunostaining (red). Increased co-localization was observed in MFP-treated tumors (yellow); left bar = 300 µm; right bar = 50 µm.