Figure 3. A demonstrates cellular growth inhibition (80-90%) following the transfection of BRM in the Rhabdoid cell lines G401, KD and KPMRT-AN over a period of 5 days.
B illustrates the induction of BRM protein in Rhabdoid cell lines, G401, KD and KPMRT-AN, following treatment with 250nM of Flavopiridol for 72 hours. “UnT” denotes the untreated parental cell line, and “FP” denotes the Flavopiridol-treated cell lines. H460 was used as the positive control, and GAPDH was used as the loading control. C and D demonstrate the induction of BRM protein in G401and KD cell lines, respectively, following a 72-hour treatment with 3µM flavonoids from each of the six flavonoid structural groups [1: Luteolin (flavone), 2: Quercetin (flavonol), 3: Genistein (isoflavone), 4: Hespiridin (flavanone), 5: EGCG (flavanol), and 6: Delphinidin (anthocyanin)]. UnT denotes the untreated parental cell line, and H460 was used as the positive control. GAPDH was used as the loading control. E The Rhabdoid cell lines, G401, KD and KPMRT-AN, harboring either scrambled shRNA (grey bar), or anti-BRM shRNA (black bar), were treated with 250nM of Flavopiridol. Daughter cell lines harboring the scrambled shRNA elicited considerable growth inhibition (65-70%) over 5 days following the treatment with Flavopiridol. In comparison, growth inhibition was significantly attenuated (20-30%) in cell lines harboring the anti-BRM shRNA (p<0.05). F demonstrates the reduction in the level of phospho-Rb in the G401 and KD cell lines following the treatment with (1) 250nM of Flavopiridol, (2) 3µM Luteolin, and (3) 3µM Quercetin for 72 hours. “UnT” denotes the untreated parental cell lines. GAPDH was used as the loading control.