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. 2014 May 4;5(10):3316–3332. doi: 10.18632/oncotarget.1945

Figure 6. A illustrates the induction of BRM mRNA by 5-7-fold as measured by qPCR in 4 Rhabdoid cell lines, G401, KD, KPMRT-AN and LM, following transfection of BAF47.

Figure 6

B demonstrates cellular growth inhibition (~80%) following the transfection of BAF47 in Rhabdoid cell lines, G401, KD, TM87 and KPMRT-AN, over a period of 5 days. C shows the level of BRM mRNA following the gene-specific shRNA-mediated knockdown of BAF47 in two BRM-positive, non-Rhabdoid cell lines, H441 and H460. No significant changes in BRM mRNA were observed between the daughter cell lines harboring the scrambled shRNA or the anti-BAF47 shRNA (p>0.05). D shows the G401, KD, TM87 and KPMRT-AN cell lines which harbor either scrambled shRNA (scr-shRNA) or anti-BRM shRNA and that were also transduced with BAF47. Daughter cell lines harboring the scrambled shRNA elicited growth inhibition (~70-80%) over a period of 5 days following the transfection of BAF47. In comparison, growth inhibition was significantly attenuated (~25-30%) in cell lines harboring the anti-BRM shRNA (p<0.05). E demonstrates the reduction in the phospho Rb level in G401 and KD cell lines following the transduction of BAF47. “UnT” denotes the untreated parental cell lines. GAPDH was used as the loading control. F G401 and KD cell lines harboring either scrambled shRNA (scr-shRNA) or anti-BRM shRNA were transduced with the short form (S-BAF47) or with the long form (L-BAF47) of BAF47. Daughter cell lines harboring the scrambled shRNA (scr-shRNA) elicited appreciable growth inhibition (~75-80%) over a period of 5 days following the transfection of either L-BAF47 or S-BAF47. The S-BAF47 transduced into the daughter cell line harboring antiBRM shRNA showed a greater degree of growth inhibition (~50%; p<0.05) than same cell line transduced with the L-BAF47 (~25%).

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