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. 2014 May 13;5(10):3386–3398. doi: 10.18632/oncotarget.1960

Figure 4. Effect of galectin-3 depletion on the expression of neogenin-1 in human gastric cancer cells.

Figure 4

(A) mRNA and protein levels of galectin-3 and neogenin-1 after transfection of AGS cells with scrambled siRNA (scRNA), galectin-3 siRNA, or neogenin-1 siRNA. β-actin was used as a loading control. (B) Expression levels of galectin-3 and neogenin-1 in SNU-638 cells as determined by western blotting after transfection with pcDNA3.1/NT-GFP-galectin-3 and vector control of pcDNA3.1/NT-GFP. (C-D) After transfection of AGS cells with scrambled siRNA (scRNA), galectin-3 siRNA, or neogenin-1 siRNA, the migration and invasion assay were performed as described in “Methods” and quantification of (C) cell migration (*, ** p<0.0001) and (D) cell invasion are presented as a histogram (*, ** p<0.0001). All experiments were performed in triplicate.