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. 2014 Jan 29;28(4):299–308. doi: 10.7555/JBR.27.20130072

Fig. 5. The effect of TLR2 and TLR4 on Mφ polarization induced by different S. japonicum antigens.

Fig. 5

A: Percentage of CD16/32+ or CD206+ cells among F4/80+ cells as determined by FACS after NCA, SWAP and SEA stimulation when the TLR2 or TLR4 pathway was blocked (compared with the isotype control, ∗P < 0.01). B: IL-12 and IL-10 cytokine levels in the supernatant of RAW264.7 cultures by ELISA after NCA, SWAP and SEA stimulation when the TLR2 or TLR4 pathway was blocked (compared with the isotype control, ∗P < 0.01). C: Relative transcription level of inos and arg1 level in RAW264.7 by RT-PCR after NCA, SWAP and SEA stimulation when the TLR2 or TLR4 pathway was blocked (compared with the isotype control, ∗P < 0.01). The results are shown as mean of 3 independent experiments.