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. 2014 Jul 18;4:5749. doi: 10.1038/srep05749

Figure 1. Clioquinol induces LC3-II up-regulation in both leukemia and multiple myeloma cells.

Figure 1

(A), the chemical structures of chloroquine (CHQ), clioquinol (CLQ) and nitroxoline (NXQ). (B), leukemia cell line K562 and multiple myeloma cell line RPMI-8226 were treated with CHQ, CLQ, or NXQ for 12 h, cells were collected and prepared for whole cell lysates followed by immunoblotting against LC3. (C), MM cell lines (RPMI-8226 and OPM2) and leukemia cell lines (OCI-AML2, THP-1 and K562) were treated with clioquinol (CLQ, 0 or 20 μM) for 24 h, cell lysates were then prepared for immunoblotting analysis against LC3. (D), K562 and LP1 cells were exposed to increasing concentrations of CLQ for 24 h followed by LC3 against specific antibodies. (E), OCI-AML2 and LP1 cells were treated with 20 μM of CLQ for 6, 12 or 24 h, followed by LC3 analysis. GAPDH was used as an internal control.