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. 2014 Jul 18;4:5737. doi: 10.1038/srep05737

Figure 4. Visualization of sequencing read depth in SeqMonk for three amplicons previously used for comparing enzymes.

Figure 4

Left column shows amplicons BRCA1.1, BRCA1.6 and BRCA2.8 and their boundaries. Right column shows junction regions. Arrows indicate boundaries. Coverage is log2-transformed. The coverage plot demonstrates that significant variations of read depth may still exist within and between amplicons from long-range PCR. Loss of coverage may occur at junction of two amplicons (B), but can be recovered by larger overlapping of two amplicons (D, F).