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. 2014 Jul 18;4:5750. doi: 10.1038/srep05750

Figure 2. Macrophages secrete inflammatory cytokines in response to breast-cancer derived exosomes in vitro.

Figure 2

(a) Cytokine array identifies IL-6, CCL2, TNFα, and GCSF as the inflammatory cytokines whose expression is most highly induced following exposure to MDA-231 and MCF7 exosomes, but not MCF10A exosomes. The original full-length blots are shown. (b–d) Quantitative RT-PCR for IL-6 and TNFα in (b) RawkB cells, (c) THP-1 cells, and (d) bone marrow derived macrophages following administration of exosomes derived from MDA-231 (231) and MCF10A (10A) cells. (e) Quantitative RT-PCR for IL-6 and TNFα in RawkB cells pre-treated with 40 μM Bay 11-7082 for 1 h followed by stimulation with 231 exosomes for 6 h. *p < 0.001.