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. 2014 Jun 19;111(2):309–317. doi: 10.1038/bjc.2014.203

Figure 3.

Figure 3

Nimocinol acetate induces autophagy markers in BCa cells. (A and B) To determine the dose- and time-dependent effects of NA on expression of autophagy markers in MCF-7 cells, whole-cell lysates were used for western blot analysis to determine the expression levels of Atg3, Atg5, Atg7,Atg12, Beclin 1, LC3A, and LC3B after NA treatment. (C and D) Similarly, dose- and time-dependent effects of NA on expression of autophagy markers were determined in MDA-MB-231 cells. (E) The dual-luciferase reporter assay showing LC3B promoter activity in NA-treated MCF-7 and MDA-MB-231 cells. *P<0.05 and ***P<0.0001.