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. 2014 Jun 19;111(2):309–317. doi: 10.1038/bjc.2014.203

Figure 6.

Figure 6

Nimocinol acetate-induced autophagy is LC3B-dependent. (A) MCF-7 cells were transiently transfected with scrambled control (siScr) or LC3B-specific (siLC3B) siRNA for 36 h followed by treatment with NA. Cells were then harvested and cell viability was measured. Whole-cell lysates were prepared and western blot was performed to assess the levels of LC3B and β-actin proteins. (B) Similarly, MDA-MB-231 cells were transiently transfected with siScr or siLC3B, and cell viability and protein expression levels were measured. Data are expressed as the mean±s.e.m. of two independent experiments. *P<0.05, **P<0.005 and ***P<0.0001.