Skip to main content
. 2014 Jun 3;111(2):375–385. doi: 10.1038/bjc.2014.281

Figure 4.

Figure 4

DCA treatment induced ROS production and mTOR inhibition in cancer cells and DCA-induced autophagy is reversed by 48-h after cessation of treatment. Western blots of LC3B and α-tubulin in 24-h vehicle-treated, 24-h DCA-treated and 48-h DCA-recovered (A) HCT116 Bax-ko and (B) HCT116 WT cells. (C) Increase in reactive oxygen species (ROS) production following 1-h DCA treatment in HT29, HCT116 WT and HCT116 Bax-ko cells (mean±s.e.m. n=4 per group, *P<0.005). (D) Western blots of pS6 ribosomal protein, total S6 ribsomal protein, p4E-BP1 and β-actin (loading control) in DCA-treated HCT116 Bax-ko, HCT116 WT and HT29 cells, indicating that mTOR is downregulated.