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. 2014 Jun 10;111(2):386–394. doi: 10.1038/bjc.2014.293

Figure 5.

Figure 5

Effects of ESDN/DCBLD2 silencing by si-ESDN/DCBLD2 transfection on cancer cell lines. (A upper) ESDN/DCBLD2 mRNA expression 72 h after transfection with si-ESDN/DCBLD2. GUSB expression was used for normalisation. (A lower) ESDN/DCBLD2 protein expression 72 h after transfection with si-ESDN/DCBLD2. GAPDH was used as a loading control. (B–D) Effects of ESDN/DCBLD2 silencing on cancer cell lines. (B) Cell proliferation was determined with XTT assays in FaDu and SAS cell lines 72 h after transfection with 10 nM of si-ESDN/DCBLD2, si-control or mock transfection. (C) Cell migration activity determined by the migration assay. (D) Cell invasion activity determined by the Matrigel invasion assay. *P<0.0001.