Single ion images of biochemical species that show region specific
distributions. Positive and negative ion species show characteristic
localization patterns that are well in line with anatomical regions
of the hippocampus of 6-month old control animals. Protein specific
fragments (pos: NaKCNO, Na2CNO) were found in highest levels
in the CA 1–4 and the granular cell layer of the DG. Similar
observations were made for a yet unidentified species at m/z 91.00 in negative mode. Vitamin E (Vit.E, [M
+ H]+ 430.31), fatty acids (FA 18:1, [M – H]− 281.32) and cholesterol (Chol., [M – H]− 385.33) were localized to the molecular layer of the
DG as well as mossy fibers projecting from the DG to the CA3 (scale
bar = 1 mm, color scale indicates relative intensity in %).