Figure 3.

Single ion images of biochemical species that show region specific distributions. Positive and negative ion species show characteristic localization patterns that are well in line with anatomical regions of the hippocampus of 6-month old control animals. Protein specific fragments (pos: NaKCNO, Na₂CNO) were found in highest levels in the CA 1–4 and the granular cell layer of the DG. Similar observations were made for a yet unidentified species at m/z 91.00 in negative mode. Vitamin E (Vit.E, [M + H]⁺ 430.31), fatty acids (FA 18:1, [M – H]⁻ 281.32) and cholesterol (Chol., [M – H]⁻ 385.33) were localized to the molecular layer of the DG as well as mossy fibers projecting from the DG to the CA3 (scale bar = 1 mm, color scale indicates relative intensity in %).