Figure 8.

Immunohistochemistry of hippocampus. Double antigen staining was performed against GFAP and S100 beta on hippocampal sections of control (A) and BMAA exposed animals (B). The data show massive gliosis and reactive astrocyte species localizing to the histopathologically altered region in the hippocampus of BMAA-treated rats. This was indicated by increased GFAP immune-reactivity (red) in the CA1 of BMAA exposed animals (B−D) compared to controls (A). (C) Magnification of outlined area indicated in (B). GFAP positive cells (C) were also found to stain positively for S100 beta serving as additional marker for astrocyte activation (B,D). Magnifications: (A,B) lens × 10; (C,D) lens × 20.