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HEK 293T cells were transiently transfected with GPR88 and GloSensor cAMP construct (A) or GloSensor cAMP only (B). 2-PCCA (1), 2, and 3 inhibited isoproterenol-induced cAMP production in GPR88 cells, but not in the control cells. The data are the means of quadruplicate measurements with standard deviation shown as error bars and are representative of at least three independent experiments.
