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. 2014 Nov 15;61(100):579–586. doi: 10.1016/j.bios.2014.06.011

Fig. 3.

Fig. 3

EMSA titrations of wt SSB (A), SSBG23CC51V (B) and SSBG23CC51V-FDA5M (C) in a solution of dT70 (2 .5 pM) at 200 mM NaCl. The concentration range (0.8, 1.4, 2.3, 3.9, 6.5, 10.8, 18 and 50 nM) was the same in all gels and lanes labelled C correspond to control reactions in the absence of SSB proteins. Data from the SSBG23CC51V-FDA5M binding (duplicate gels) were fitted to a one site specific binding with Hill slope equation resulting in Kd of 1.17 nM.