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. Author manuscript; available in PMC: 2014 Jul 18.
Published in final edited form as: Cell Rep. 2013 Jun 27;4(1):189–204. doi: 10.1016/j.celrep.2013.05.043

Figure 2. The Global Profiles of H3K4me3 and H3K27me3 in QSCs and ASCs.

Figure 2

(A) Immunofluorescence of Pax7 and H3K4me3 on freshly isolated single fibers. The arrows indicate a fiber-associated QSC that is positive for both Pax7 and H3K4me3.

(B) Distribution of H3K4me3 around the TSSs in QSCs and ASCs. Normalized tag intensity of H3K4me3 3 kb upstream and downstream of the TSSs across the genome is shown in the plot.

(C) Venn diagram of genes marked by H3K4me3 at their TSSs in QSCs and ASCs.

(D) H3K4me3 and H3K27me3 distribution at the TSS of genes that expressed at high levels in QSCs but were downregulated in ASCs. The H3K4me3 and H3K27me3 profiles of representative genes are shown in the top panels, and the level of changes in their expression upon SC activation revealed by microarray analysis is shown in the bottom bar graphs. Error bars represent SDs.

(E) Distribution of H3K27me3 around the TSSs in QSCs and ASCs. Normalized tag intensity of H3K27me3 3 kb upstream and downstream of the TSSs across the genome is shown in the plot.

(F) Immunofluorescence of freshly isolated myofibers with Pax7 and H3K27me3 antibodies (top panels) and fibers cultured for 2 days ex vivo with MyoD and H3K27me3 antibodies (bottom panels). Images were acquired with the same exposure and gain. Fiber-associated SCs are indicated by the arrows, and all other DAPI+ nuclei are myonuclei within the fiber.

See also Figures S3 and S4 and Table S2.