Fig. 3.

Fusion of GFP at the C terminus of the KSHV SCP supports self-assembly. Sf21 cells were co-infected with baculoviruses expressing all six capsid proteins. The ORF65-expressing virus was tagged at the C terminus with HA or EGFP and at the N terminus with EGFP. The capsid band that was observed by incident light following sucrose gradient sedimentation of infected cell lysates was photographed. Capsid assembly was evident only with the CHA- and CGFP-tagged SCP fusions. The arrow indicates the direction of sedimentation.